Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus
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DNA Extraction of Almond without Phenol and Liquid Nitrogen
Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...
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Abstract Background and objectives: Genomic DNA extraction of bacterial cells is of processes performed normally in most biological laboratories therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA extraction of Staphylococcus aureus was investigated using some laundry detergent brands available in Iran to achieve ...
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Recently different methods are introduced for DNA extraction from bacteria. Bacterial DNA can be extracted using a commercial kit and conventional (e.g., boiling, phenol-chloroform and detergent methods). In the manual methods, chemically solution such as EDTA, SDS, NaCl, Tris hydrochloride, phenol-chloroform, proteinase K and lysozyme can be proposed (1). The phenol-chloroform DNA extraction m...
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عنوان ژورنال
دوره 11 شماره None
صفحات 926- 929
تاریخ انتشار 2012-10
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